The effect of Pasteurization Milk storage to Quality Microbe Thermoduric and Chemical properties

Novita Dewi Kristanti


The aim of this study was to determine the quality of thermoduric bacteria and chemical quality of pasteurized milk stored with different time periods. The material was milk from the farmers that was pasteurized at 950C for 21 seconds. The research Method used completely randomized design. The variables observed were TPC, Koliform, Kapang, E. coli bacteria, fat content, protein content, lactose content, total solid, and solid non fat (SNF). The results of the study showed that E. coli and Koliform were not found in pasteurized milk, but a number of thermoduric microbes were  found. Pasteurized milk has a shelf life of 30 days under 40C ± 10C conditions and has no significant changes in fat content, lactose levels, non-fat dry matter content and protein content.


A shelf life; Thermoduric bacteria

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Alvarez, V. B. and F. Parada-Rabell. 2014. Health Benefitf=s, Risks, and Regulation of Raw and Pasteurized Milk. Extension FactSheet. Ohio State University Extension’s. Diunduh tanggal 13 juli 2014.

Anema, S. G. 2008. Heat and/or High-Pressure Treatment of Skim Milk: Changes to the Casein Micelle Size, Whey Proteins and the Acid Gelation Properties of the Milk. International Journal of Dairy Technology. 61: 245–252.

Anonymous. 1995. Susu Pasteurisasi. SNI 01-3951-1995. Standar Nasional Indonesia. Badan Standardisasi Nasional.2010. FDA Rekommended Pasteurization Time/ Temperatur. Penn State University.

Barraquio, V. L. 2014. Which Milk is Fresh. International Journal of Dairy Science & Processing. Vol 1 (2): 1 – 6.

Chapman, K. W., and K. J. Boor. 2001. Acceptance ff 2% Ultrapasteurized Milk by Consumers, 6 to 11 Years Old. J. Dairy Sci. 84: 951–954.

Chavan, R. S., S. R. Chavan, C. D. Khedkar. and A. H. Jana. 2011. UHT Milk Processing and Effect of Plasmin Activity on Shelf Life: A Review. Comprehensive Reviews in Food Science and Food Safety. 10: 251-268.

Claeys, W.L., S. Cardoen., G. Daube., J. De Block., K. Dewettinck., K. Dierick., L. De Zutter., A. Huyghebaert., H. Imberechts., P. Thiange., Y. Vandenplas. and L. Herman. 2012. Raw or Heated Cow Milk Consumption: Review of Risks and Benefits. Food Control 31: 251- 262.

Claeys, W.L., C. Verraes., S. Cardoen., J. De Block., A. Huyghebaert., K. Raes., K. Dewettinck. and L. Herman. 2014. Consumption of Raw or Heated Milk from Different Species: An Evaluation of the Nutritional and Potential Health Benefits. Food Control 42: 188 – 201.

Gandy, A. L., M. W. Schilling, P. C. Coggins, C. H. White, Y. Yoon. and V. V. Kamadia. 2008. The Effect of Pasteurization Temperature on Consumer Acceptability, Sensory Characteristics, Volatile Compound Composition, and Shelf-Life of Fluid. J. Dairy Sci. 91: 1969-1777.

Hassan, N. B. A., M. O. M. Abdalla. and A. A. A. M. Nour. 2009. Microbiology Quality of Heat-Treated Milk During Storage. Pakistan Journal of Nutrition. 8 (12): 1845 – 1848.

Herzallah, S. M., M. A. Humeid. and K. M. Al-Ismail. Effect of Heating and Processing Methods of Milk and Dairy Products on Conjugated Linoleic Acid and Trans Fatty Acid Isomer Content. J. Dairy Sci. 88:1301–1310

Juffs and Deeth. 2007. Scientific Evaluation of Pasteurisation for Pathogen Reduction in Milk and Milk Products. Food Standars Australia New Zealand.

Kristanti, N. D., Rosyidi, D., Radiati, L. E and Purwadi. 2015. Phylogenetic Tree and Heat Resistance of Thermoduric Bacteria Isolated from Pasteurization Milk in Indonesia. International Journal of Biosciences. 6 (11): 87-98.

Kristanti, N. D., Warnaen, A dan Daning, D. R. A. Titik Kontrol Kristis pada Pengolahan Susu Pasteurisasi di Koperasi Unit Desa (KUD) Dau Kabupaten Malang. Sains Peternakan 15 (1): 1-7.

Ranieri, M. L., J. R. Huck, M. Sonnen, D. M. Barbano. and K. J. Boor. 2009. High Temperature, Short Time Pasteurization Temperatures Inversely Affect Bacterial Numbers during Refrigerated Storage of Pasteurized Fluid Milk. J. Dairy Sci. 92: 4823–4832.

Reale, S., A. Campanella, A. Merigioli. and F. Pilla. 2008. A Novel Method for Species Identification in Milk and Milk-Based Products. The Journal of Dairy Research. 75: 107–112.

Rehman, Z. U. and A. M. Salariya. 2005. Effect of Storage Condition on Nutritional Quality of UHT Processed Buffalo Milk. Journal Chemical Science. 27 (1): 73 – 76.

Ruegg, P. L. and D. J. Reinemann, 2002. Milk quality and mastitis test. University of Wisconsin Extension Services. The Bovine Prac, 36,1-13.

Santos, M. V., Y. Ma. and D. M. Barbano. 2003.Effect of Somatic Cell Count on Proteolysis and Lipolysis in Pasteurized Fluid Milk during Shelf-Life Storage. J. Dairy Sci. 86: 2491–2503.

Souci, S. W., W. Fachmann. and H. Kraut. 2008. Food Composition and Nutrition Tables. (7thed.).Stuttgart:Wissenschaftliche Verlagsgesellschaft mbH: 1364.

Steel, R. G. D. dan J. H. Torrie. 1989. Prinsip dan Prosedur Statistika; Suatu Pendekatan Biometrik. Gramedia. Jakarta.

Sudarwanto, M. 2012. Pemeriksaan Susu dan Produk Olahannya. IPB Press. Institut Pertanian Bogor. Bogor.

Thevenieau, F and J. Nicaud. 2012. Microorganisms as Sources of Olis. Oilseed & Fats Crops and Lipid. 6(D603): 2 – 8.

Valik L., F. Gorner, and D. Laukova. 2003. Growth Dynamics of Bacillus cereus and Shelf-Life of Pasteurised Milk. Czech Journal Food Science.. 21: 195–202.



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