Kadar Low Density Lipoprotein dan Gambaran Histopatologi Hepar pada Tikus Model Diabetes Mellitus Tipe 1 dengan Pemberian Ekstrak Etanol Curcuma longa L

Authors

  • Herlina Pratiwi Program Studi Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Brawijaya
  • Djoko Winarso Program Studi Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Brawijaya
  • Nunung Handoyo Program Studi Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Brawijaya

DOI:

https://doi.org/10.21776/ub.jitek.2016.011.01.1

Keywords:

Diabetes mellitus type 1, etanol extract of turmeric, Histopathology of liver and LDL

Abstract

This study was conducted to determine levels of LDL and liver damage in rats (Rattus norvegicus) models of type 1 diabetes mellitus inducted by streptozotocin (STZ) with etanol extract of turmeric (Curcuma Longa L) therapy. Animals used rat (Rattus norvegicus) 3-month-old males who were divided into 5 groups, each group consisting of four mice. The group was divided according to treatment: negative control (not induced by STZ), the positive control group (STZ induced), groups of rats DM 1 with etanol turmeric extract therapy a dose of 1.2 g / kg, groups of rats DM 1 with etanol turmeric extract therapy a dose of 1.8 g / kg, and groups of rats DM 1 with etanol turmeric extract therapy a dose of 2.7 g / kg. LDL levels measured by direct method and the severity of liver damage was observed through histopatology picture. The results showed that the etanol extract of turmeric dose of 2.7 g / kg in a rats model of type 1 diabetes mellitus can lower LDL levels up to 59.55%, and reduced the severity of fatty liver with reduced fat vacuoles. The conclusion from this study that the etanol extract of turmeric contains antioxidants that can lower LDL levels and reduced the severity of fatty liver in type 1 diabetes mellitus.

References

Angulo, 2002. Nonalcoholic Fatty Liver Disease. N Engl J Med 2002: 3469 (16): 1221-31.

Barik, R., S. Jain, D. Qwatra, A. Joshi, G.S. Tripathi and R. Goyal. 2008. Antidiabetic Activity of Aqueous Root Extract of Ichnocarpus Frutescens in Streptozotocinnicotinamide Induced type II diabetes in rats. Indian Journal of Pharmacology 40(1): 19-22.

Botham, K.M., and Mayes, P.A., 2009. Pengangkutan dan Penyimpanan

Lipid. Jakarta: EGC. Hal: 225-238.

Fall, T.,Hamlin, H.H., Hedhammar, A., Kampe, O., and Egenvall, A., 2007. Diabetes Mellitus in a Population of 180.000 Insured Dogs: Incidence, Survival, and Breed Distribution. J Vet Intern Med 21:1209-1216.

Ganong, William. F. 2002. Buku Ajar Fisiologi Kedokteran; editor edisi bahasa indonesia, H.M. Djauhari Widjajakusumah-ed.20. EGC. Jakarta.

Gusti, I. A. 2013. Suplementasi Kombinasi Tempe M-2 dengan Wortel (Daucus carrota) Meningkatkan HDL dan Antioksidan Total, serta Menurunkan LDL, F2-isoprostan dan IL-6 pada Tikus Wistar Aterosklerosis [Disertasi]. Program Pascasarjana. Universitas Udayana.

Hoenig, M. 2002. Comparative Aspects of Diabetes Mellitus in Dogs and Cats. Molecular and Endocrinology 197: 221-229.

Kamagate A, Shen Qu, German P, Dongming Su, Dae Hyun Kim, Sandra S, Marcia M, and Henry D. 2008. FoxO1 Mediates Insulindependent Regulation of Hepatic VLDL Production in Mice. J Clin Invest. 2008; 118(6):2347-2364

Kohli K., Ali J., Ansari M.J., and Raheman Z. 2004. Curcumin: A Natural Antiinflammatory Agent. Indian Jurnal of Pharmacology. 37(3): 141-147

Lennernas H, Fager G. (1997). Pharmacodynamics and Pharmacokinetics of the HMGCoA Reductase Inhibitors. Clin. Pharmacokinet 1997; 403-425.

Mayfield J. 1998. Diagnosis and Classification of Diabetes Mellitus: New Criteria. American Family Phyician 58 (6): 1-8

Nugroho, A. E. 2006. Animals Models Of Diabetes mellitus: Pathology and Mechanism of Some Diabetogenics. Laboratorium Farmakologi Dan Toksikologi, Bagian Farmakologi dan Farmasi Klinik, Fakultas Farmasi Universitas Gajah Mada Yogyakarta.

Priyadarsini, K.I., Maity, D.K., Naik, G.H., Kumar, M.S., Unnikrishnan, M.K., Satav, J.G., dan Mohan, H. 2003. Role of Phenolic OH and Methylene Hydrogen on The Free Radical Reactions and Antioxidant Activity of Curcumin. Free Radical Biology and Medicine, 35(5): 475– 484.

Setiawan, B., dan Ernawati. 2007. Efek Proteksi dari Curcumin Terhadap Sel Endothelium pada Stress. Department Of Pharmacology, Faculty of Medicine Wijaya Kusuma University, Surabaya.

Wijaya A. Parameter Risiko Penyakit Vaskuler Aterosklerotik Koroner dan Serebral. Forum Diagnosticum. 1995; 3: 1-15.

Sharma, R.A., A. J. Gescher, W. P. Steward. 2005. Curcumin: The Story So Far. European Journal of Cancer, 41:1955–1968.

Sudiono, J. B., Kurniadhi, Hendrawan, A., dan Djinantoro, B. 2003. Ilmu Patologi. Penerbit EGC

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Published

2017-02-27

Issue

Vol. 11 No. 1 (2016)

Section

Articles

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