Effect of Temperature and Duration of Gasbio Heat Energy on Bee Pollen Bioactive Compounds and Bee Propolis Extraction Trigona sp

Yasri Yasri Rahmawati; Mochammad Junus

doi:10.21776/ub.jitek.2024.019.01.4

Authors

Yasri Yasri Rahmawati Animal Production Department. Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Malang, East Java, 65145, Indonesia
Mochammad Junus Animal Production Department. Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Malang, East Java, 65145, Indonesia

DOI:

https://doi.org/10.21776/ub.jitek.2024.019.01.4

Keywords:

Activity antioxidant IC50, bee bread, propolis, proteins, total phenolics

Abstract

Processing of dried bee pollen and propolis extraction to optimize the use of processed by-products of Trigona sp bees in the general public by utilizing biogas as heat energy. This research uses bee pollen and raw bee propolis (Trigona sp). The aim of the research was to determine the effect of temperature and length of time for drying bee pollen and propolis extraction on bioactive compounds. The research method used a completely randomized design (CRD) with 5 treatments and 3 replications, consist with (1) Bee pollen drying temperature with sunlight, 45 ^oC, 55 ^oC, 65 ^oC, and 75^oC, (2) Long oven time 3 days, 2,5 hours, 2 hours, 1 hour, and 15 minutes. Meanwhile, the treatment for propolis extraction with distilled water solvent is (1) Temperature 70^oC, 75^oC^, 80^oC, 85^oC, and 90^oC, (2) Length of extraction time is 220 minutes. Data were analyzed using Variant ANOVA. If there is a significant difference and effect (P<0.05) or highly significant (P<0.01), then proceed with least significant difference test. Dried bee pollen research results the best bioactive compounds were obtained at a temperature of 65 ^oC (T3) with protein content (14.09 %), total phenol (1.314 mgGAE/g), and antioxidant content (53.97 ug/mL). The best results for propolis extraction were at a temperature of 70^oC (T0) with antioxidants (21.15ug/mL) and total phenolics (0.357 mgGAE/g). Gasbio can be used as an alternative heat for drying bee pollen and propolis extraction, but when use gasbio must determine the right temperature and time.

References

Agus, A., Agussalim, N., Umami, N., & Budisatria, I. G. S. (2019_. Evaluation of antioxidant activity, phenolic, flavonoid and vitamin C content of several honeys produced by the Indonesian stingless bee: Tetragonula laeviceps. Livest Res Rural Dev, 31 (10), 152.

Anis, U., Silsia, D., & Kusumaningtyas, R. N. (2021). Pengaruh variasi pollen terhadap karakteristik kimia roti lebah. Jurnal Teknologi Agro-Industri, 8 (2),111-124.

Baliyan, S., Mukherjee, R., Priyadarshini, A., Vibhuti, A., Gupta, A., Pandey, R. P., & Chang, C. M. (2022). Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molekul, 27 (4), 3-19.

Bogdanov, S. (2016). Beeswax. Switzerland: Hexagon.

Djakaria, S. A., Batubara, I., & Raffiudin, R. (2020). Antioxidant and antibacterial activity of selected Indonesian honey against bacteria of acne. Jurnal Kimia Sains dan Aplikasi, 23 (8), 267-275.

Handayani, T. H., Budiman, M., Amalia, R. L., Pribadi, A., & Rabeca, R. (2022). Aktivitas antioksidan, total fenolik, dan total flavonoid madu Apis mellifera dari hutan akasia (Accacia crassicarpa) Riau, Indonesia dengan beberapa perlakuan pengeringan. Jurnal Biologi Indonesia, 18 (2), 231-243.

Harvi, S. P., Lipoeto, N. I., & Hidayati. (2014). Perbandingan efektivitas berkumur menggunakan propolis lebah 12.38% (Apis Mellifera) dan klorheksidin glukonat 0.2% terhadap indeks plak. Andalas Dental Journal, 2 (1), 34-43.

Isik, A., Ozdemir, M., & Doymaz, I. (2019). Effect of hor air drying on quality characteristics and physicochemical properties of bee pollen. Food Sciences and Thecnology, Campinas, 39 (1), 224-231.

Isik, A., Ozdemir, M., & Doymaz, I. (2019). Infrared drying of bee pollen: effects and impacts on food components. Czech Journal of Food Sciences, 37 (1), 69-74.

Kapoor, R., Ghosh, P., Tyagi, B., Vijay, V. K., Vijay, V., Thakur, I. S., Kamyab, H., Nguyen, D. D., & Kumar, A., (2020). Advances in biogas valorization and utilization systems: a comprehensive review. Journal of Cleaner Production, 273, 123052.

Khairunnisa, K., Mardawati, E., dan Putri, S. H. (2020). Karakteristik fitokimia dan aktivitas antioksidan ekstrak propolis lebah Trigona sp. Jurnal Industri Pertanian, 2(1),124-129.

Kunarto, B., & Sani, E. Y. (2020). Ekstraksi buah parijoto (Medinilla speciosa blume) berbantu ultrasonik pada berbagai suhu, waktu dan konsentrasi pelarut etanol. Jurnal Teknologi Pertanian, 21 (1), 29-38.

Mohdaly, A. A., Mahmoud, A. A., Roby, M. H., Smetanska, I., & Ramadan, M. F. (2015). Phenolic extract from propolis and bee pollen: composition, antioxidant and antibacterial activities. Journal of Food Biochemistry, 39 (5), 538-547.

Rismawati, S. N., & Ismiyati, I. (2017). Pengaruh variasi pH terhadap kadar flavonoid pada ekstraksi propolis dan karakteristiknya sebagai antimikroba. Jurnal Konversi, 6 (2), 89-94.

Rosyidi, D., Radiati, L. E., Minarti, S., Mustakim, M., Susilo, A., Jaya, F., & Azis, A. (2018). Perbandingan sifat antioksidan propolis pada dua jenis lebah (Apis mellifera dan Trigona sp.) di Mojokerto dan Batu, Jawa Timur, Indonesia. Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK), 13 (2), 108-117.

Safitri, E., & Purnobasuki, H. H. 2022. Aplikasi Madu sebagai Aktivator STEM CELL. Surabaya: Airlangga University Press.

Styana, U. I. F., Widodo, G. N., & Cahyono, M. S. (2022). Potensi campuran kotoran sapi dan limbah cair rumah pemotongan ayam sebagai sumber energi penghasil biogas. Jurnal Offshore: Oil, Production Facilities and Renewable Energy, 6 (1), 29-38.

Spulber, R., Dogaroglu, M., Babeanu, N., & Popa, O. (2018). Physicochemical characteristics of fresh bee pollen from different botanical origins. Romanian Biotechnological Letters, 23 (1), 13357-13365.

Vassev, K. K., Olczyk, P., Kafmierczak, J., Mencner, L., & Olczyk, K. (2015). bee pollen: chemical composition and therapeutic application. Evidence-Based Complementary and Alternative Medicine,1 (1), 1-6.

Wibawanti, J. M. W, Mudaawaroch, R. E., & Pamungkas, S. (2020). Inovasi pengolahan produk turunan madu lebah klenceng menjadi bee pollen kapsul sebagai sumber antioksiidan di Desa Jelok Kecamatan Kaligeding, Purworejo. Surya Abdimas, 4 (1), 19-24

Zahra, N. N., Muliasari, H., Andayani, Y., & Sudarma, I. M. (2021). Analisis kadar fenolik total dan aktivitas antiradikal bebas madu dan propolis Trigona sp. asal Lombok Utara. Analit: Analytical and Environmental Chemistry, 6 (1), 74-82.